|
KMID : 0604520190450010037
|
|
Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 1 p.37 ~ p.47
|
|
|
Studies on Antioxidant, Anti-inflammatory and Whitening Effects of Oriental Herbal Extracts (Mix) including Eucommiae cortex
|
|
Choi Da-Hee
Kim Mi-Ran
Kim Min-Young
Kim Ho-Hyun
Park Sun-Young
Hwang Hyeon-Shik
|
|
|
Abstract
|
|
|
|
Recently, due to the increase in skin diseases caused by particulate matter, endocrine disruptor and environmental changes, the trend of development of cosmetic materials has been shifting to the more safe and effective ingredients based on natural materials rather than existing synthetic compounds like steroids and antihistamines. This study aimed to develop a new natural cosmetic materials using oriental herbs such as Eucommiae cortex, Alpinia oxyphylla Miquel and Bombyx batryticatus. First, DPPH assay was performed to examine the antioxidative activity of the herbal extract (Mix) and 98.8% DPPH radical scavenging activity was confirmed at 400 μg/mL concentration of it. In order to confirm the whitening efficacy of oriental herbal extracts(mix), the amount of melanin synthesized after stimulation of α-MSH with B16F10 cells was measured. Results showed that it was decreased to 27.1% comparing with the only α-MSH treated group, which confirmed the whitening efficacy. Also, both nitric oxide(NO) production and iNOS and COX-2 expression were significantly reduced in RAW264.7 macrophages activated by LPS in the presence of the extracts(Mix). The mRNA expression of the inflammatory cytokines such as IL-1α, IL-1β, IL-6, and TNF-α was also analyzed to confirm the inhibition effect of the extracts on inflammation. Finally, to confirm the enhancement of skin barrier function, the expression of claudin 1 gene, a tight junction protein, was observed using human keratinocyte HaCaT cells and increased as concentration dependent manner. From these results, it is concluded that the oriental herbal extracts(Mix) containing Eucommiae cortex, Alpinia oxyphylla Miquel and Bombyx batryticatus is effective for antioxidant, anti-inflammation, skin whitening, and skin barrier and thus could be applied as a new natural cosmetic material.
|
|
|
KEYWORD
|
|
|
Eucommiae cortex, Alpinia oxyphylla Miquel, Bombyx batryticatus, anti-inflammation, whitening efficacy, claudin 1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|